WWL 2023 Preliminary program

Wednesday Feb 1, 2023: Wet Lab Day 1

Location: lecture room CH1-222 and adjoining labs
08:45 Regisration
9:00 Welcome and introduction / Split into groups for labs
10:30 coffee break
11:00 Labs: Introduction to laboratory practice and DNA isolation
12:00 lunch
13:00 Labs: Extraction of HMW (high molecular weight) DNA from yeast
(coffee break when convenient)
18:00 city (student guided tour, dinner on your own)

Thursday Feb 2, 2023: Wet Lab Day 2

Location: lecture room CH1-222 and adjoining labs
9:00 All hands meeting, progress presentations, day overview
9:30 Labs: Extraction of HMW DNA from yeast (cont.)
(coffee break when convenient)
12:30 lunch
14:00 Excursion to the University Research Park and Geneton
(coffee break when convenient)
18:00 dinner / evening on your own

Friday Feb 3, 2023: Wet Lab Day 3

Location: lecture room CH1-222 and adjoining labs
9:00 All hands meeting, progress presentations, day overview
9:30 Sample QC (electrophoresis, Nanodrop, Qubit)
10:30 Labs: Purification of HMW DNA by anion-exchange chromatography (Qiagen 100/G)
(coffee break when convenient)
12:30 lunch
13:30 Labs: Sample QC (electrophoresis, Nanodrop, Qubit)> (coffee break when convenient)
18:00 dinner / evening on your own

Saturday Feb 4, 2023: Wet Lab Day 4

Location: lecture room CH1-222 and adjoining labs
9:00 All hands meeting, short progress presentations, day overview
9:30 Labs: Preparation of sequencing library (ligation kit LSK110) and the library QC (Qubit)
Loading the library onto a MinION flow-cell
(coffee break when convenient)
13:30 lunch
14:30 Labs: Preliminary data analysis
16:30 coffee break
17:00 Labs summary meeting
19:00 dinner / evening on your own

Sunday Feb 5, 2023: Social, Analysis Day 1

9:00-17:00 Trip and social: Danubiana Meulensteen Art Museum / Čunovo water dam, Lunch, Bratislava post-communism tour
dinner in town on your own

evening program in UniSpace in mathematics building
weekend / evening access through physics building
19:45 Registration
20:00 Welcome / Introduction to the Analysis Workshop
Analysis group leader presentations
Split into analysis groups

Monday Feb 6, 2023: Analysis Day 2

Location: UniSpace in mathematics building, lectures in lecture room C
09:00-10:00 Lecture: Jozef Nosek - Diversity of Yeast Genomes: A Glimpse at the Lodderomyces/Candida Clade
10:00 coffee break
work in groups
12:30 lunch
work in groups
16:30 coffee break
17:00-18:00 Lecture: Miroslava Slaninova - Introduction to ethics in biomedical research
18:00-19:00 dinner (on site)
19:00 dissemination / communication (Nadia Pisanti)
19:30 day 1 analysis summary presentations

Tuesday Feb 7, 2023: Analysis Day 3

Location: UniSpace in mathematics building, lectures in lecture room C
09:00-10:00 Lecture: Peter Celec - On the extras of extracellular DNA
10:00 coffee break
work in groups
12:30 lunch
work in groups
13:30-14:30 Lecture: Travis Gagie - Pangenomic FM-indexes
16:30 coffee break
17:00-18:00 Lecture: Leszek Pryszcz - On genome assembly
18:00-19:00 dinner (on site)
19:00 day 2 analysis summary presentations

Wednesday Feb 8, 2023: Analysis Day 4

Location: lecture room C
09:00-10:00 Lecture: Vladimir Boza - Processing nanopore signal
10:00 coffee break
work in groups
12:30 lunch
work in groups
16:30 coffee break
17:00 day 3 analysis summary presentations
closing remarks / future work plan